SC assembly in A) wild-type, B) SA RNAi, C) Nipped-B RNAi, D) SA86 germline clone and E) c(2)M mutant oocytes. Meiotic prophase begins in region 2a, with zygotene and early pachytene oocytes, with later pachytene stages in regions 2b and 3. Shown are representative oocytes in region 2a and region 3, with C(3)G (green) and centromere histone CID (red) and DNA (blue). An arrow indicates synapsis at the centromeres as shown by colocalization of C(3)G and CID. The scale bars = 5μm. See also Figure S1 and Figure S3.