Figure 5. Meiotic cohesin protein C(2)M is incorporated throughout pachytene.

(A–D) C2M-HA was induced with a 1 hour heat shock and then fixed 6 or 24 hours later. In all images, the DNA is in blue, C(3)G is in green, C(2)M-HA is in red and the scale bar = 5 μm. Interpreting these experiments takes advantage of the germarium organization, which contains approximately seven 16-cell cysts, each containing an oocyte. The cysts are arranged in temporal order, where the more posterior oocytes (e.g region 3) have been in pachytene longer than more anterior ones (e.g. region 2a) and it takes about 12–24 hours for a cyst to move down one position (Figure S4). All oocytes carried the UASp-c(2)M^3XHA transgene either in a wild-type (A,B) or in a c(2)M mutant (C,D) background. C(2)M-HA was induced with a 1 hour heat shock and then fixed 6 (A) or 24 (B,D) hours later, or without heat shock (C). (E,F) Oocytes from the indicated region with C(2)M-HA induced by arm-GAL4 in either wild type (E) or a c(2)M mutant (F) background. See also Figure S4 and Figure S5.