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. Author manuscript; available in PMC: 2017 Jul 12.
Published in final edited form as: Circulation. 2016 Jun 28;134(2):141–152. doi: 10.1161/CIRCULATIONAHA.115.019692

Figure 3. Elevated Angiopoietin-2 in serum from patients with LVADs induces angiogenesis in human endothelium.

Figure 3

Figure 3

(a) Human umbilical vein endothelial cells were assayed on Matrigel and incubated overnight with serum from patients with HF, LVAD, or OHT (n=17, 35, and 14 respectively) in the presence or absence of an Ang-2 blocking antibody. Tubule formation was then quantified visually by an investigator blinded to sample identity. Tubule formation was significantly greater in cultures treated with serum from patients with LVADs compared with controls. Co-treatment with Ang-2 blocking antibody significantly reduced angiogenic growth in cells treated with serum from patients with LVADs but had no significant effect in cells treated with serum from HF or OHT patients. † p<0.01 (b) Among LVAD patients, tubule formation correlated strongly with serum Ang-2 concentration. R2=0.645, p<0.001