Fig. 3.

SOD1 protein does not affect the voltage dependence of inactivation of Na_V1.3-β1 channel complexes. a Na_v1.3 current was recorded by step depolarizations to –30 mV from a conditioning test potential between –120 and 0 mV, in 20 mV steps (scale bar x = 0.2 ms, y = 1 μA). b Inactivation curves were calculated from the data in a. The solid lines represent Boltzmann fits to the data. The average midpoint of inactivation (V_1/2) for control (–61.55 ± 0.82, n = 19), hSOD1^WT (–64.24 ± 1.82, n = 17) and hSOD1^A4V (–61.89 ± 0.95, n = 13) and the voltage dependency (slope) of inactivation curves for control (–4.88 ± 0.39, n = 19), hSOD1^WT (–5.00 ± 0.31, n = 17) and hSOD1^A4 (–5.32 ± 0.40, n = 13) were similar (p > 0.05, ANOVA with Tukey’s post test)