Table 2.
BtCepA β-lactamase activity after mannan induction.
| Straina | Description | Mannan (mg/l) | nmol/mg/min | Fold-Inductionc |
|---|---|---|---|---|
| GH359 | WT (P3784_low) | 0 | 304.5 ± 41.3 | – |
| 0 [Cef5]b | 642.9 ± 70.9 | – | ||
| GH360 | ΔBtcepA (P3784_low) | 50 | 3.4 ± 2.0 | – |
| GH361 | ΔBtcepA (P3784_low::BtcepA) | 0 | 11.4 ± 0.3 | 1.0 |
| 50 | 131.7 ± 4.6 | 11.6 | ||
| 100 | 177.1 ± 26.9 | 15.6 | ||
| 250 | 191.1 ± 25.5 | 16.8 | ||
| GH364 | ΔBtcepA (P3784_med::BtcepA) | 0 | 1016.9 ± 13.4 | 89.4 |
| 5 | 5872.3 ± 350.4 | 516.4 | ||
| 50 | 13133.2 ± 503.7 | 1154.9 |
a
The strains are also described in Table 1.
b
Addition of 5 mg/l cefotaxime.
c
Induction factor = the mean value obtained in the indicated conditions/the mean value obtained for the non-induced GH361 strain (11.4).