Table 1.

Characterization of EAEC strains solated in the study.

Daycare no.	Child	Age in years	Sample no.(1-6)	Sample ID	Serotype	EAEC genesa	Phylogenetic group	Symptoms reported	Foreign travelb/destination
1	A	3	3	C 506-10-1115c	O111:H21	aatA, aggR, aaiC	B1	Diarrhea Abdominal pain
2	B	1	1	C 135-10-1029d	O86:H30	aatA, aggR	D	Vomiting Reduced appetite	Norway
3	Cef	3	1	C 198-10-1076	O3:H2	aatA, aggR, aaiC	A	Reduced appetite
			3	C511-10-1175	O3:H2	aatA, aggR, aaiC	A	None
			5	C 72-11-1284	O3:H2	aatA, aggR, aaiC	A	Vomiting Reduced appetite
4	D	4	4	C 876-10-1231d	O111:H21	aatA, aggR, aaiC	B1	Diarrhea Vomiting Reduced appetite
5	Eg	4	3	C 896-10-1202	O99:H4	aatA, aggR, aaiC	A	None
5	Fg	2	3	C 380-14-1203	O99:H4	aatA, aggR, aaiC	A	None
5	G	7	5	C 74-11-1288-Ai	O92:H33	aatA, aggR, aaiC	A	Abdominal pain reduced appetite
				C 75-11-1288-B	O92:H33	aatA, aggR, aaiC	B1
5	H	6	5	C 77-11-1289-Ai	O?:H-	aatA	A	Diarrhea Abdominal pain Reduced appetite
				C 78-11-1289-B	Orough:H-	aatA	B1
6	I	1	5	C 25-11-1270	O127:H21	aatA, aggR, aaiC	A	N/A
7	Jh	2	2	C 880-10-1242d	O78:H2	aatA, aggR, aaiC	A	Vomiting	Sweden
7	Kh	5	2	C 884-10-1241d	O78:H2	aatA, aggR, aaiC	A	None	Sweden
8	L	1	6	C 831-11-1405	O92:H33	aatA, aggR, aaiC	A	Diarrhea Reduced appetite	Macedonia
9	M	3	6	C 707-13-1630-Ai	O14:H-	aatA, aggR, aaiC	A	N/A
				C 27-13-1630-B	(O48), O62:H-	aatA, aggR, aaiC	B1
10	N	6	1	C 409-12-1496	O127:H21	aatA, aggR, aaiC	B1	None
11	O	5	1	C 404-12B-1446	O15:H-	aatA, aggR, aaiC	A	N/A
			6	C 725-13-1750c	O14:H-	aatA	A	Diarrhea Nausea Abdomin al pain
12	P	2	1	C 249-13-1451	O86:H2	aatA, aggR, aaiC	A	None	N/A
			4	C 31-13-1641	O17:H18	aaiC	D	None	Turkey
13	Q	3	1	C 407-12-1453c	O44:H18	aatA, aggR, aaiC	D	None
14	R	1	1	C 250-13-1460d	O9:H21	aatA, aggR, aaiC	B1	Diarrhea Vomiting	Tanzania
15	S	4	2	C 383-14-1508d	O114:H21	aaiC	A	None
			3	C 1028-12-1564d	O114:H32	aaiC	A	Vomiting
15	T	2	2	C 1030-12-1570-Acdi	O23:H-	aatA, aggR, aaiC	B1	None
				C 1031-12-1570-Bc	O55:H10	aatA, aggR, aaiC	B1
15	U	2	5	C 35-13-1659	O103:H43	aatA	A	None	Turkey
16	V	1	2	C 413-12-1554d	O153:H30	aatA, aggR	D	Vomiting
17	Wj	2	3	C 374-14-1618d	O?:H34	aatA, aggR, aaiC	D	None	Seychelles
			4	C 34-13-1657&d	O?:H34	aatA, aggR, aaiC	D	Nausea Vomiting
			6	C 724-13-1748d	O?:H34	aatA, aggR, aaiC	D	None
19	X	2	5	C 73-13-1690-Adi	O?:H30	aatA, aggR	D	Reduced appetite	Egypt
				C 719-13-1690-Bd	O153:H30	aatA, aggR	D
20	Y	N/A	N/A	C 382-14-1422	O125:H21	aatA, aggR, aaiC	B1	N/A	N/A

Abbreviations: EAEC, enteroaggregative Escherichia coli, aatA, gene encoding the dispersin transporter protein, aggR, gene encoding a transcriptional activator, aaiC, gene encoding a secreted protein.

^aDetected by PCR and confirmed by DNA hybridization.

^bForeign travel reported within the last 2 months prior to stool sampling.

^cCo-infection with AEEC.

^dMulti-drug-resistant EAEC strain.

^eCarrier of the same EAEC strain for a year.

^fUse of antibiotics.

^gSiblings.

^hSibling.

ⁱInfected with two different EAEC strains at the same point of observation.

^jCarrier of the same EAEC strain for 10 months.

N/A not available.