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. 2016 Jul 13;7:1098. doi: 10.3389/fmicb.2016.01098

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Copyright © 2016 Weiland-Bräuer, Kisch, Pinnow, Liese and Schmitz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of immobilized MBP-QQ-2 on biofilm formation of K. oxytoca. Purified MBP-QQ-2 was covalently linked to slide surfaces of flow cells using concentrations of 2.5 fmol/mm² – 2.5 pmol/mm². 1.35 × 10⁸ cells of K. oxytoca were supplemented and after 1 h, flow cells were flowed for 72 h at 30°C with 20 mL/h GC medium. Biofilms were stained with Syto9. Images were recorded with Leica TCP Confocal Laser Scanning Microscope (Leica) and analyzed with Leica software. 3D CLSM images showing live cells stained with Syto9 (green) were scanned along the biofilm depth (z-axis). Scale μm.