Fig. 2.

Drug testing comparing static and perfusion cultures. a Representative images of spheroids after 72 h of treatment with different drugs at the concentration of 10 µM. Note the significant morphological difference between static and perfused paclitaxel treatment. b Cell viability testing comparing perfusion and static spheroids. The drug concentration was 10 µM, after 72 h of treatment. A significant difference was observed between 3D static and perfusion testing of paclitaxel (p = 0.00312 by Student’s t test). c Representative MPM images of spheroids after treatment with different drugs. As shown in a and c, the spheroids perfused with paclitaxel totally lost the spheroid structures after 72 h. Scale bar 100 µm