Figure 2.

Corrinoid and benzimidazole lower ligand concentrations in B₁₂-unamended (A) and B₁₂-amended (B) enrichments at the end of 13–18 days’ subculturing cycle, error bars represents standard deviation, n=3 (¹Abiotic control without exogenous vitamin B₁₂; ²Abiotic control with 74 nM (c.a.100 µg/L) vitamin B₁₂; Note: (A) and (B) share the same legend, but different y axis scales. Most of the cobalamin in SANASB12 was detected in the supernatant, while most corrinoids were detected in the cell pellets of the other enrichments. Lower ligands were mostly detected in the supernatant).