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. 2016 Jul 11;6:29703. doi: 10.1038/srep29703

Figure 4. Gene expression of Smad1, 5 and 8/9 and co-Smad4.

Figure 4

Box plots represent qRT-PCR values given as mean normalised expression relative to 18S rRNA and the untreated control (reference line) using an efficiency corrected formula (n = 6). Stars indicate significant differences to the untreated control. (A) Expression of Smad1 was increased only at 4 h of BMP-7 stimulation (*p = 0.002). (B) Smad5 expression was not regulated compared to unstimulated control. (C) Smad8/9 expression increased after 4 h in the high BMP-7 group and highly increased after 8 h in both concentrations (*p = 0.002). (D) Smad4 expression was slightly increased after 1, 4 and 8 h of stimulation (*p = 0.002).