Table 2. Primer Sequences.
| Gene | Accession number | Primer sequence Forward | Primer sequence Reverse | Efficiency |
|---|---|---|---|---|
| 18S | NM_022551 | CGGAAAATAGCCTTTGCCATC | AGTTCTCCCGCCCTCTTGGT | 1.79 |
| BMPR-Ia | NM_004329.2 | TGCTGGACGAAAGCCTGAAC | TTCCACGATCCCTCCTGTGA | 1.78 |
| BMPR-Ib | NM_001203.2 | ATGCCACCACCATTGTCCAG | GTGACCACAGGCAACCCAGA | 2.05 |
| ActR-I | NM_001111067.2 | GTCGGGAAAGGCAGGTATGG | TCCGTTTCCCTGAACCATGA | 1.93 |
| BMPR-II | NM_001204 | GCTCTTGCCGTCTTGCTCAT | GGCGCACCAGTCTATTTCCA | 1.93 |
| ActR-IIA | NM_001278580 | TTGCGGGGATTGTCATTTGT | GGAGAAGGTGGGGGTGGTC | 2.03 |
| ActR-IIB | NM_001106 | CTGCACTGCTACGCCTCCTG | ACACCTGGGGGTTCTCCTCA | 1.98 |
| Smad1 | NM_005900.2 | GAAGCGTTCCATGCCTCCTC | GGCATACACCTCCCCTCCAA | 1.75 |
| Smad4 | NM_005359 | TCCCAACATTCCTGTGGCTTC | CTGCTGCTGTCCTGGCTGA | 2.00 |
| Smad5 | NM_001001420.1 | TTCCACCAGCCCAACAACAC | GGCAGGAGGAGGCGTATCAG | 1.91 |
| Smad8/9 | NM_001127217 | ACACCACCCCTGCCTTATCA | CCTGGAATGTCTCCCCAACTC | 1.81 |
| ID1 | NM_181353 | GCTGCTCTACGACATGAACG | CCAACTGAAGGTCCCTGATG | 1.87 |
| OC | NM_199173.4 | TGAGAGCCCTCACACTCCTC | CGCCTGGGTCTCTTC ACT AC | 1.78 |
| Col-IA1 | NM_000088.3 | TGACCTCAAGATGTGCCACT | ACCAGACATGCCTCTTGTCC | 1.92 |
| Col-IIA1 | NM_033150.2 | CGCACCTGCAGAGACCTGAA | TCTTCTTGGGAACGTTTGCTGG | 1.87 |
| Runx2 | NR_103532 | GCCCCCAAACAGTATCTTGA | GCCTGAAGTGAGGTTTTAGGC | 1.86 |
| Aggrecan | NM_001135.3 | CCAGTGCACAGAGGGGTTTG | TCCGAGGGTGCCGTGAG | 1.89 |
| Mohawk | NM_173576.2 | TGGTTTGCTAATGCAAGACG | CCTTCGTTCATGTGGGTTCT | 1.85 |
| Osterix | NM_001173467.2 | CACCCACCTCAGGCTATGCT | TGGATGCCTGCCTTGTACCA | 2.08 |
| Sox9 | NM_000346 | GACTCGCCACACTCCTCCTC | CTCAGCTCGCCGATGTCCA | 1.84 |
| Scleraxis | Commercial product. QuantiTect Primer Assay (Qiagen) | |||