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. 2016 Jul 11;6:29766. doi: 10.1038/srep29766

Figure 6. Validation of RNA-binding proteins with a western blot analysis.

Figure 6

(A) Western blots showing (i) total soluble protein (TSP) from Arabidopsis Col 0 ecotype, TSP from Landsberg erecta (Ler-0), RBP non UV (nUV)-crosslinked Col 0, RBP nUV-crosslinked Ler-0, RBP UV-crosslinked Col 0, RBP UV-crosslinked Ler-0 and RNAse treated samples from Col 0 and Ler-0 and (ii) RNA-RNA-binding protein (RNA-RBP) complex, RNA extract, RBP UV-crosslinked samples probed with antibodies against the DEAD box-6 helicase (DDX6). (B) Western blot showing (i) RNA-RBP complex, RNA extract, RNAse treated sample, TSP, TSP sample after extracting RBP (TSP-RBP), RBP nUV-crosslinked and RBP UV-crosslinked samples and (ii) RNA-RBP complex, RNA extract and RBP UV-crosslinked samples probed with antibodies against the clathrin heavy chain 1 (AT3G11130). (B) (i) and (ii) are two independent runs performed to check reproducibility of the validation experiment. (C) Western blot showing RNA-RBP complex, RNA extract and RBP UV-crosslinked samples probed with antibodies against catalase 3 (AT1G20620).