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. 2016 Jul 12;6:29424. doi: 10.1038/srep29424

Figure 3. Microglia activation is responsible for the anorexia and body weight loss induced by TLR2 in the mouse hypothalamic Arc.

Figure 3

(A) Representative images (3–4 mice analyzed) show immunosignals of Iba-1 in the hypothalamic Arc. Scale bar = 100 μm (20 μm for higher magnification view in inset). (B,C) Increased intensity of Iba-1 signals in the Arc (B) of icv Pam3CSK4 (Pam3)-injected mice was significantly attenuated by preadministration of minocycline (Mino), a bacteriostatic antibiotic that effectively inhibits microglia activation. There was no difference in the number of microglia (C) among different treatment groups (CTL, n = 8 section/4 mice; Pam3, n = 6 section/3 mice; Mino + Pam3, n = 8 section/4 mice; ** P < 0.01, ***P < 0.0001 by unpaired two-tailed Student’s t-tests; NS, not significant). (D,E) Pam3-induced anorexia (D) and weight loss (E) were significantly mitigated by administration of Mino for 3 consecutive days prior to icv Pam3 injection. (CTL, n = 5 mice; Pam3, n = 6; Mino, n = 7; Mino + Pam3, n = 7; *P < 0.05, **P < 0.01, ***P < 0.001 by two-way ANOVA; NS, not significant). All data are presented as mean ± s.e.m.