Figure 7.

PCB extract contributed to an expanded population of inducible T regulatory cells. CD4⁺CD62L⁺ naïve T cells from splenocytes and mLN cells from BALB/c mice were cultured with 10 μg/mL plate-bound anti-CD3 mAb and 2 μg/mL soluble anti-CD28 mAb in the presence of 0–10 μg/mL PCB. After three days, the levels of IFN-γ (a), IL-4 (b), IL-17 (c), and TGF-β (d) in the culture supernatants were measured using ELISA. Data are presented as the mean ± SD of triplicate determinations and were analyzed using ANOVA followed by F-protected Fisher's least significant difference test. ^∗ P < 0.05; ^∗∗ P < 0.01 versus OVA control. (e) The expression of Treg-associated molecules was compared between the sham and experimental groups. GranB: Granzyme B. Data are presented as the mean ± standard deviation (SD) of triplicate determinations and were analyzed using unpaired t-test. ^∗ P < 0.05 and ^∗∗ P < 0.01 versus OVA control.