Table 1. Apparent affinity of CNG channels containing different subunit composition.
| Constructs | cGMP (μM) | |||||
|---|---|---|---|---|---|---|
| EC50 | H | EC50,l | H1 | EC50,h | Hh | |
| CNGA2 | 1.74 | 1.9 | ||||
| CNGA2RE* | 606.7 | 2.7 | ||||
| CNGA2TM* | 10.4 | 2.3 | ||||
| CNGA2:A4 | 1.28 | 1.7 | ||||
| CNGA2:B1b | 1.9 | 2.0 | ||||
| CNGA2:A4:B1b | 0.76 | 2.0 | ||||
| CNGA2RE:A4RE:B1b | 177.8 | 1.2 | 3.6 | 1** | ||
| CNGA2RE:A4:B1b RE | 364.2 | 1.1 | 8.86 | 1** | ||
| CNGA2RE:A4:B1b | 733.2 | 1.2 | 5.7 | 1.1 | ||
*from Nache et al., 2012.
**H was set to unity.
Fit parameters for concentration-activation relationships of CNG channels containing different compositions of subunits with either functional or disabled binding domains. The relationships for the constructs 1 to 6 were fitted with Equation 1 and for the constructs 7 to 9 with Equation 2.