Figure 1. Tah18 and Dre2 are regulated by Yap1.

(A) DRE2 and YAP1 overexpression can rescue viability of tah18 cells upon HU chronic exposure. 10-fold serial dilutions of yeast cells grown until log phase were spotted on YPD medium containing increasing concentration of HU as indicated. Growth was assessed after 4 days at 28 °C. (B) Average expression of DRE2 and TAH18 mRNA levels in three strains with increasing YAP1 expression levels. mRNA levels have been quantified in three strains: no YAP1 expression (yap1Δ), wild-type YAP1 expression (WT) and YAP1 overexpression (YAP1 carried out on a multicopy plasmid). Increasing expression of YAP1 in the three strains is illustrated as a triangle. mRNA level of each gene was calculated as ratio relative to that of the wild-type (WT) strain (set as 1). (C) Dre2 and Tah18 protein levels in three strains with increasing YAP1 expression levels. Yeast cells were treated with 100 mM HU for 1 hour and collected for protein analysis. Dre2 and Tah18 were detected with specific antibodies and actin was used as an internal control. Odyssey infrared imaging system was used for quantification. Relative Dre2 or Tah18 amounts were calculated as follow: Dre2 or Tah18 amounts = [Dre2 or Tah18 band intensity]/[Act1 band intensity]. Amounts in WT were set as 1. The reported values are the means of three independent experiments ± SEM. P values were calculated using unpaired one-tailed t-test, n = 3.