Figure 2. YAP1 or DRE2 overexpression improves tah18-5I5 cytosolic Fe-S biosynthesis and hydroxyurea block release.

(A,C) Isopropylmalate isomerase (Leu1) activity measurement. Citraconate was used as a substrate of Leu1. Leu1 activity (nmol mg⁻¹ min⁻¹) was calculated from the decrease in OD at 235 nm due to citraconate isomerization. The genotype of yeast strains used is indicated. The reported values are the means of three independent experiments ± SEM. (B) Flow cytometry analysis of yeast cells incubated in the presence of HU (20 and 40 mM as indicated). The genotype of yeast strains used is indicated. Time points at 0, 1, 2, 3 and 4 hour exposure are shown. S phase restart is indicated by an arrow in tah18-5I5 cells overexpressing YAP1 or DRE2.