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. 2016 Jul 11;6:29419. doi: 10.1038/srep29419

Figure 7. Narrowing down a Treg cell-stimulating domain on the ppinsΔA12-21 antigen.

Figure 7

(a) Aminoacid sequences of ppinsΔA12-21, ppins1–36, ppins15–66, ppins1–89 and ppins75–99 are shown. PD-L1−/− mice were vaccinated with pCI/ppins1–36 (b) (n = 3), pCI/ppins15–66 (c) (n = 3) or pCI/ppins1–89 (d) (n = 4) followed by an injection of the diabetogenic pCI/ppins vector after 12 days. (e,f) PD-1−/− mice (n = 4) were vaccinated (at day 0 and day 17) with pCI/Core-ppins75–99 encoding a chimeric fusion protein of the HBV Core antigen and ppins77–99 (e) or recombinant rCore-ppins75–99 particles adsorbed to alum (f) followed by an injection of the diabetogenic pCI/ppins DNA. Arrows indicate the injection of the diabetogenic pCI/ppins DNA. Cumulative diabetes incidences (%) were determined by regular blood glucose measurements.