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. 2016 Jul 13;6:29673. doi: 10.1038/srep29673

Figure 1. Gas6 treatment enhances expression of LXRα and LXRβ and their target genes in BMDM.

Figure 1

Mouse BMDM were stimulated with 400 ng/ml Gas6, 1 μM T0901317, 10 ng/ml interferon (IFN)-γ, 10 ng/ml IL-4, or 100 ng/ml LPS for 4 h (a,b) or 400 ng/ml Gas6 for the indicated times (c–g). (a,b,e) The amounts of the LXRα, LXRβ, ABCA1, ABCG1, ApoE, AIM, Arg2, VEGF, YM1, and Arg1 mRNAs were analyzed by real-time PCR and normalized to that of Hprt mRNA. (c,d,f,g) The relative abundances of LXRα, LXRβ, ABCA1, ABCG1, ApoE, Aim, Arg2, and VEGF proteins were determined by Western blotting analysis. The relative densitometric intensity was determined for each band and normalized to β-actin. Data in all bar graphs are means ± SEM of three independent experiments. *P < 0.05 compared with control.