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. 2016 Jul 13;6:29521. doi: 10.1038/srep29521

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Immunohistochemical fluorescent analysis of a paraffin-embedded section of invasive ductal carcinoma tissue was conducted by dual staining with primary rabbit anti-human CD68 and mouse anti–human sCLU, or rabbit anti-human CD33 and mouse anti-human sCLU, followed by anti-mouse Alexa-647 and anti-rabbit Alexa-488.