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. 2016 Jul 13;6:29710. doi: 10.1038/srep29710

Figure 4. Overexpression of wild type EphA7 or stimulation with ephrin A5 increases while protein kinase-deficient EphA7 reduces gephyrin clustering.

Figure 4

(a,c,e) After cotransfection of pEGFP-N1 and wildtype EphA7 (wt-EphA7) or dominant-negativ EphA7 (dn-EphA7), cells were stained for gephyrin (yellow, a’,c’, e’) or PSD95 (red, a”,c”, e”). Magnifications of insets in (a-g) are shown in (a’–g’), and in (a”–e”), respectively. All images were rendered using Imaris software. Scale bars, 10 μm. (b,d) control and wt-EphA7 transfected cells were stained for the γ2 subunit of GABAA receptors. (f,g) Neurons transfected with wt-EphA7 were treated with DMSO or rapamycin/DMSO (rapa) and subsequently stained for gephyrin. (h) The densities of gephyrin, γ2 subunit of GABAA receptors and PSD95 punctae were quantified in transfected neurons as identified by EGFP fluorescence. wt-EphA7: gephyrin, n = 50; GABAA γ2, n = 45; PSD95, n = 30. dn-EphA7: gephyrin, n = 30, ***p < 0.0001, ANOVA, Fisher-PLSD. Error bars, S.E.M.