Fig. 1.

Screening strategy for library molecules. A total of 1200 US Food and Drug administration (FDA)-approved small molecules were screened for intracellular growth inhibition of Cryptococcus neoformans in macrophages. The primary screen yielded 19 active compounds that were further probed for host cell cytotoxicity and fungal growth inhibition. Eleven compounds were shortlisted for phagosomal maturation screening, which identified only one promising drug hit candidate, D9 (fendiline hydrochloride). Finally, the mechanism of action of fendiline was investigated. MoA, mode of action.