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. 2016 Jul;48(1):69–77. doi: 10.1016/j.ijantimicag.2016.04.018

Fig. 5.

Fig. 5

Dose-dependent effect of D9 on phagosomal maturation and intracellular killing. (A) Phagosomal acidification at differential doses of D9 quantified by LysoTracker® Red staining after treatment with the respective drug dose for 18 h. Values represent the mean ± standard deviation (S.D.) collected from observing 300–400 phagosomes at each time point for each treatment across three to five biological repeats (Fisher's exact test, ***P < 0.001). (B) Effect of respective doses of D9 on intracellular viability of H99-GFP in J774A.1 murine macrophages as quantified from time-lapse movies. The intracellular proliferation rate is calculated as t12/t0 ratio, where t12 and t0 indicate the number of internalised cryptococci at 12 h and 0 h post-infection, respectively; n = 3 (one-way ANOVA + Tukey post-test, ***P < 0.001; NS, not significant). NT, untreated; DMSO, dimethyl sulphoxide; AmB, amphotericin B; CCP, cryptococcal-containing phagosome.