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. 2016 Jul 12;16:455. doi: 10.1186/s12885-016-2483-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Giemsa staining after Ad-delE1B55 infection. Cells were treated with ZOL (MSTO-211H: 10 μM, NCI-H226: 60 μM, NCI-H28: 80 μM) and/or Ad-delE1B55 (MSTO-211H: 2 × 10³ vp/cell, NCI-H226: 1 × 10³ vp/cell, NCI-H28: 2 × 10³ vp/cell) for 72 h. Nuclear morphological changes were examined after the Giemsa staining. In Ad-delE1B55-treated cells, small and highly condensed nucleus (solid arrow) and large and uncondensed nucleus (open arrow) were detected. An upper panel and lower panel are x4 and x10 in magnifications, respectively