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. 2016 Jul 12;16:455. doi: 10.1186/s12885-016-2483-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Expression of Ad receptors and infectivity of Ad. a Expression levels of CAR and integrin αvβ3 or αvβ5 molecules on MSTO-211H and NCI-H28 cells were analyzed with flow cytometry. Representative profiles of cells untreated or treated with ZOL (MSTO-211H: 10 μM, NCI-H28: 80 μM) for 48 h are shown. CAR expression on MSTO-211H cells was always biphasic and both low and high CAR-positive cells were detected Shaded profiles are those stained with 2nd Ab alone. b Efficacy of Ad infection examined with Ad-GFP. Cells were infected with Ad-GFP (300–3,000 vp/cell) and treated with or without ZOL (MSTO-211H: 10 μM, NCI-H28: 80 μM) for 48 h. The mean fluorescent intensity of the GFP-positive cells was analyzed with flow cytometry and expressed with an arbitrary unit. Averages and the SE bars are shown (n = 3)