Table 3.
Expression of Ad receptor molecules after ZOL treatments
| Cells | Ab | ZOL treatment | Mean fluorescent intensity (average ± SE) | % radio (average ± SE) |
|---|---|---|---|---|
| MSTO-211H | 2nd Ab alone | (−) | 12.42 ± 0.07 | |
| (+) | 13.44 ± 0.07 | 108.19 ± 0.51 | ||
| CAR | (−) | 95.21 ± 2.04 | ||
| (+) | 95.86 ± 0.09 | 100.69 ± 0.09 | ||
| Integrin αvβ3 | (−) | 13.59 ± 0.02 | ||
| (+) | 14.67 ± 0.04 | 107.92 ± 0.04 | ||
| Integrin αvβ5 | (−) | 15.64 ± 0.10 | ||
| (+) | 18.12 ± 0.03 | 115.88 ± 0.64 | ||
| NCI-H28 | 2nd Ab alone | (−) | 11.39 ± 0.03 | |
| (+) | 11.55 ± 0.86 | 101.38 ± 0.79 | ||
| CAR | (−) | 127.26 ± 0.31 | ||
| (+) | 119.29 ± 0.69 | 93.73 ± 0.03 | ||
| Integrin αvβ3 | (−) | 1006.61 ± 1.24 | ||
| (+) | 718.29 ± 2.33 | 71.36 ± 0.12 | ||
| Integrin αvβ5 | (−) | 44.01 ± 0.09 | ||
| (+) | 22.01 ± 0.03 | 50.01 ± 0.13 |
Cells untreated or treated with ZOL (MSTO-211H: 10 μM, NCI-H28: 80 μM) for 48 h were subjected to flow cytometry to detect expression of the cellular receptors of type 5 Ad. Means fluorescence intensity was expressed as an arbitrary unit and influence of ZOL on the expression levels was expressed as a percent ratio based on respective ZOL-untreated cases as a control (n = 3)