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. 2016 Jul 1;13:175. doi: 10.1186/s12974-016-0640-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Confocal microscopy revealed hippocampal neurodegeneration in rats after long-term THP treatment. Hippocampal neuronal loss and neurodegeneration were detected using NeuN and FJB, fluorescent markers for neurons and neurodegeneration, respectively. a Confocal microscopy shows colocalization of some neurons with neurodegeneration. Scale bar = 50 μm. b Integrated immunofluorescence density analysis was performed using ImageJ. THP-treated rats show slightly increased FJB-labeled cells in selected hippocampal areas. ^* P < 0.05 compared with the NS-treated aging controls (Student’s t test)