Table 3.

PCR detection of P. gingivalis, T. denticola and T. forsythia genomic DNA in infected B10.RIII mice tissues

Group	Polybacterial infection	Positive systemic tissue samples
		Heart	Liver	Spleen	Kidney	Lung	Knee joint
		n = 10	n = 10	n = 5	n = 10	n = 10	n = 10
I	Pg/Td/Tf	4/3/1	2/3/1	0/0/0	5/4/2	2/0/0	0/0/0
II	Pg/Td/Tf + collagen	5/2/0	3/2/1	0/0/0	4/5/2	3/1/0	2/0/0
III	Collagen control	0/0/0	0/0/0	0/0/0	0/0/0	0/0/0	0/0/0
IV	Sham infection	0/0/0	0/0/0	0/0/0	0/0/0	0/0/0	0/0/0

After 24 weeks of gingival infection, heart, liver, spleen, kidney, lung, and hind limb ankle joint tissues were harvested from B10.RIII mice and extracted genomic DNA were subjected to 40 cycles of PCR analysis using species-specific primers for the three oral bacteria P. gingivalis (Pg), T. denticola (Td), and T. forsythia (Tf). The numbers indicated with forward slash correspond to the number of mice positive for Pg/Td/Tf genomic DNA, respectively