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. 2016 Jul 12;16:452. doi: 10.1186/s12885-016-2516-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Silibinin induced apoptosis through mitochondrial ROS in PC-3 cells. a PC-3 cells were treated with 150 μM silibinin for indicated times. MMP was determined using fluorescence dye DiOC₆ by flow cytometry. b Protein expression was analyzed by western blotting with antibodies for pro-caspase-3 and PARP. β-Actin was used as a loading control. c Apoptosis was analyzed after treatment with 150 μM silibinin for 48 h with the presence or absence of 0.5 μM DPI by flow cytometry. d Protein expression was analyzed by western blotting. β-Actin was used as a loading control. Data are presented as mean ± SD (n = 3 in each group). ^* p <0.001 vs. the control group