Fig. 5.—

The assembly of FdeC is enhanced by the TAM. (A) The domain architecture of FdeC is represented based on PDB structures and homology-modeled domains. The predicted overall structure of intact FdeC is colored based on the sequence conservation between FdeC subtypes (blue = 95% sequence identity; red = 60% sequence identity). The black lines “N” and “C” encompass the peptides recovered from the 50 kDa fragment and the 100 kDa fragment derived by proteinase K as described in the main text. (B) Escherichia coli harboring plasmid pCJS50(fdeC) were pulse labeled as in D. Mass spectrometry defined the identity of the 50 kDa N-terminal fragment of FdeC (N), and the 100 kDa C-terminal fragment of FdeC (C).