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. 2016 Jun 11;8(6):2065–2075. doi: 10.1093/gbe/evw122

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Recombination breakpoints within M7124 capsule. (A) Sequence alignment of galE genes showing recombination breakpoint at position 858 relative to W cc22 strain, α-275. (B) Sequence alignment of cssA genes demonstrating breakpoint at position 804 relative to α-275. (C) Sequence alignment of rfbA2 and rfbC2 showing recombination breakpoint within rfbA2 position 634. (D) Recombination breakpoints detected by at least one of seven methods included in RDP v4.56. The topmost horizontal bar represents M7124 cps sequence; three black arrows correspond to locations of breakpoints identified in (A–C) above; asterisk marks location of multiple small (0.1–1.5 kb) putative mosaic recombinant segments that were not supported by visual examination of aligned sequences (see main text). Topmost horizontal bar represents W cc11; colored boxes represent donor sequences from Y cc23 (green), W cc22 (blue) and C cc22 (red).