Table 5.

Grouping of pRK415 sigma library members.

No.	Member	Description	42°C survive	Mean integral value			BLASTP		Growth group
				30°C	42°C	42°/30°	Identity %	coverage %
1	rpoD	σ1RpoD-σNCRσ2RpoD-σ3RpoD-σ4RpoD	yes	17.38	16.46	0.947	100	100	high
2	sigAmut03	σ1SigA-σNCRσ2SigA-σ3SigA-σ4SigAm3	yes	16.54	15.40	0.931	49	98	high
3	sigAmut01	σ1SigA-σNCRσ2SigA-σ3SigA-σ4SigAm1	yes	17.67	15.32	0.867	48	98	high
4	chim06	σ1SigA-σNCRσ2RpoD-σ3RpoD-σ4RpoD	yes	17.31	14.94	0.863	88	98	high
5	chim09	σ1RpoD-σNCRσ2RpoD-σ3RpoD-σ4SigA	yes	18.53	15.73	0.849	98	99	high
6	sigAmut02	σ1SigA-σNCRσ2SigA-σ3SigA-σ4SigAm2	yes	19.78	16.08	0.813	48	98	high
7	chim14	σ1SigA-σNCRσ2RpoD-σ3SigA-σ4RpoD	yes	13.95	10.36	0.743	84	98	high
8	sigA	σ1SigA-σNCRσ2SigA-σ3SigA-σ4SigA	yes	16.24	10.87	0.669	48	98	medium
9	chim13	σ1RpoD-σNCRσ2SigA-σ3RpoD-σ4SigA	yes	16.93	10.68	0.631	63	99	medium
10	chim08	σ1SigA-σNCRσ2SigA-σ3RpoD-σ4SigA	yes	18.93	11.51	0.608	51	98	medium
11	chim12	σ1SigA-σNCRσ2RpoD-σ3RpoD-σ4SigA	yes	14.90	8.60	0.577	85	98	medium
12	chim05	σ1RpoD-σNCRσ2SigA-σ3SigA-σ4SigA	yes	18.62	10.55	0.567	59	99	medium
13	chim01	σ1RpoD-σNCRσ2RpoD-σ3SigA-σ4SigA	yes	15.02	8.43	0.561	94	99	medium
14	chim04	σ1SigA-σNCRσ2RpoD-σ3SigA-σ4SigA	yes	16.41	7.02	0.428	73	98	medium
15	chim10	σ1SigA-σNCRσ2SigA-σ3SigA-σ4RpoD	no	16.68	1.79	0.108	50	98	no
16	chim03	σ1RpoD-σNCRσ2SigA-σ3RpoD-σ4RpoD	no	17.26	1.79	0.104	72	100	no
17	chim11	σ1RpoD-σNCRσ2SigA-σ3SigA-σ4RpoD	no	16.4	1.00	0.061	61	100	no
18	chim07	σ1RpoD-σNCRσ2RpoD-σ3SigA-σ4RpoD	no	16.74	0.87	0.052	96	100	no
19	chim02	σ1SigA-σNCRσ2SigA-σ3RpoD-σ4RpoD	no	14.75	0.20	0.014	54	98	no
20	pRK415	empty vector	no	14.63	0.19	0.013	NA	NA	no

Integral values represent the area under the curve for each library member. RpoD sequence was used as query in BLASTP searches to determine identity percentages. Growth group determined by clustering library members. NA, not applicable.