Table 2.
D1/2 unfolding rate constants and relative abundances for SiaA and mutants.
Protein | kfast (h−1) | Abundance of the Fast Phase (%) | kslow (h−1) | Abundance of the Slow Phase (%) | D1/2 (M) |
---|---|---|---|---|---|
WT | 0.32 ± 0.02 | 15 | 0.026 ± 0.001 | 85 | 3.1 |
C47A | 0.24 ± 0.02 | 30 | 0.033 ± 0.001 | 70 | 2.9 |
C58A | 0.46 ± 0.02 | 12 | 0.010 ± 0.001 | 88 | 2.4 |
K61A | 0.41 ± 0.04 | 11 | 0.012 ± 0.001 | 89 | 2.5 |