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. Author manuscript; available in PMC: 2016 Jul 13.
Published in final edited form as: Stem Cells. 2009 Feb;27(2):375–382. doi: 10.1634/stemcells.2008-0546

Figure 4.

Figure 4

Epigenetic analysis of IGF1 promoter: ChIP assay for SD-MSCs, MSCs, and SD-MSCs after 5 days in CCM. The data are presented as the relative abundance of immunoprecipitated protein in the IGF1 promoter. (A): ChIP assay of Pol II followed by quantitative PCR for IGF1 promoter region. (B): ChIP assay of acetylated H4 followed by quantitative PCR for IGF1 promoter region. (C): Ratio of dimethylated H3K4 and trimethylated H3K9 distribution on IGF1 promoter. (D): Gel electrophoresis of the amplification product of the ChIP assay with H3K4Me and H3K9Me for SD-MSCs and MSCs. Abbreviations: CCM, complete culture medium; ChIP, chromatin immunoprecipitation; IGF1, insulin-like growth factor 1; MSC, mesenchymal stem cell; PCR, polymerase chain reaction; SD-MSC, serum-deprived mesenchymal stem cell.