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. 2016 Jun 14;1(1):68–106. doi: 10.20411/pai.v1i1.100

Figure 3.

Figure 3.

Utility of RNAscope for the detection and quantification of vRNA+ cells in a combination antiretroviral therapy (cART) SIV NHP model with strong correlation to qRT-PCR. (A) The number of vRNA+ cells quantified by RNAscope and (B) the number of vRNA copies quantified by qRT-PCR are (C) strongly correlated before and during cART. Data were log10 transformed and P values were based on associations between paired comparisons using the Pearson's Correlation test. (D) The proportions of vRNA+ cells within distinct secondary lymphoid tissue anatomical sites (i.e., B cell follicles (BCF), T cell zone, and medullary cords) before and (E) during cART. P values were based on the Mann-Whitney test. (F) The frequency of vRNA+ cells in BCF increased, while that of vRNA+ cells in T cell zone decreased. The proportion of vRNA+ cells within the MC is unchanged, suggesting a steady rate of cell trafficking. P values were based on the Wilcoxon matched-pairs signed rank test. (G) RNAscope in situ hybridization (ISH) has the ability to detect low-level vRNA+ cells (not shown) and FDC-bound viral particles in chronically SIV+ RMs before (top panel) and after 26 weeks of cART (bottom panel). Scale bars = 50 μm.