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. 2016 Jul 13;3:16046. doi: 10.1038/mtm.2016.46

Figure 3.

Figure 3

Potent GFP expression in descending motor axons in lumbar spinal cord after mid-thoracic subpial AAV9 injection in adult pigs. (a,b) Transverse spinal cord section taken from the lumbar spinal cord after subpial AAV9-UBI-GFP injection into the mid-thoracic subpial space 6 weeks prior. Intense GFP expression in transversal cut axons in lateral (LF) and ventral (VF) funiculus can be seen (white asterisks). A relatively lower density of GFP+ axons in the dorsal funiculus was identified (DF). Correspondingly, a high density of GFP+ motor axons projecting into the gray matter localized between the dorsal horn (DH) and ventral horn (VH) can also be seen. (c) A higher resolution confocal image shows very fine arborization of GFP+ axons and terminal boutons in the central gray matter (Scale bars: a-1,000 μm; c- 30 μm). DF, dorsal funiculus; DH, dorsal horn; LF-lateral funiculus; VF, ventral funiculus; VH, ventral horn.