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Retrograde and anterograde-transport-mediated GFP expression in medulla oblongata and cerebellum at 8 weeks after subpial cervical AAV9 delivery in adult rats. (a–c) An intense GFP expression in medulla oblongata representing a mixture of retrogradely and anterogradely labeled axons can be seen. A fine arborization of GFP-expressing spino-cerebellar tract terminals in the cerebellum can also be identified (a; insert) (Scale bar: a-2 mm; insert-200 μm; b, c-500 μm).
