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. 2016 Jul 13;3:16046. doi: 10.1038/mtm.2016.46

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Retrograde and anterograde-transport-mediated GFP expression in the brain stem and motor cortex at 8 weeks after subpial cervical AAV9 delivery in adult rats. (a) Intense bilateral GFP expression can be seen throughout the brain stem (NR, nucleus ruber; ML, medial lemniscus; CPD, cerebral peduncle). (b) Specific and intense GFP expression in retrogradely-labeled pyramidal neurons in the motor cortex (layer V) can be identified (MOp-primary somatomotor area; CC-corpus callosum; ACB-nucleus accumbens) (Scale bars: a-1 mm; inserts No.1 and No.2–50 μm; b-1mm; inserts No.1–250 μm; No.1-1-50 μm; No.2 and No.3–250 μm).