TABLE 5.

Interaction of osmolarity, pH and lactate Experiment 1

Treatment	Concentration			Response drop number				Mean angular response	Response
	Osmols	pH	Lactate (M)	1	2	3	4
									%
1	0.308	7.38	5 × 10−2	2	4	1	6	30.30	25.5
2	0.308	7.38	2 × 10−2	4	5	2	5	34.95	32.8
3	0.308	6.82	5 × 10−2	1	0	0	0	10.43	3.3
4	0.308	6.82	2 × 10−2	3	1	6	2	28.98	23.5
5	0.270	7.38	5 × 10−2	2	4	4	1	27.88	21.9
6	0.270	7.38	2 × 10−2	3	4	5	3	33.88	31.1
7	0.270	6.82	5 × 10−2	0	0	0	2	12.25	4.5
8	0.270	6.82	2 × 10−2	4	3	2	4	31.18	26.8

Lactate × pH two-way table (mean angular response)
Lactate (M)	pH
	6.82	7.38
2 × 10−2	30.08	34.42
5 × 10−2	11.43	29.09

Analysis of variance
Source of variation	D.F.	MS
Between drops	3	12.091
Between treatments	(7)	(360.561)
Osmolarity (O)	1	0.138
pH	1	975.716 3
Lactate (L)	1	1158.008 3
O × pH	1	28.312
pH × L	1	359.790 1
L × O	1	1.488
O × pH × L	1	0.476
Error	21	79.735
Total	31
Theoretical variance	∞	75.3

Response is the number of normal blastocysts from 12 two-cell ova after three days cultivation in vitro. Two-cell ova obtained 45 hours after HCG injection.

¹0.05 > P > 0.01.

³P < 0.001.