TABLE 7.

Interaction of osmolarity, pH and pyruvate

Treatment	Concentration			Response drop number				Mean angular response	Response
	Osmols	pH	Pyruvate (M)	1	2	3	4
									%
1	0.308	7.38	3.16 × 10−4	6	7	6	4	43.78	47.9
2	0.308	7.38	1.00 × 10−4	4	1	4	1	26.05	19.3
3	0.308	6.82	3.16 × 10−4	4	5	2	7	37.35	36.8
4	0.308	6.82	1.00 × 10−4	6	4	3	5	37.63	37.3
5	0.270	7.38	3.16 × 10−4	9	8	11	4	55.80	68.4
6	0.270	7.38	1.00 × 10−4	2	2	5	3	29.60	24.4
7	0.270	6.82	3.16 × 10−4	4	4	4	2	32.50	28.9
8	0.270	6.82	1.00 × 10−4	8	6	5	6	46.23	52.2

Pyruvate × pH two-way table (mean angular response)
Pyruvate (M)	pH
	6.82	7.38
1.00 × 10−4	41.93	27.83
3.16 × 10−4	34.93	49.79

Analysis of variance
Source of variation	D.F.	MS
Between drops	3	82.585
Between treatments	(7)	(376.563)
Osmolarity (O)	1	186.728
pH	1	1.163
Pyruvate (P)	1	447.753 1
O × pH	1	69.915
pH × P	1	1677.652 3
P × O	1	12.375
O × pH × P	1	240.354
Error	21
Total	31
Theoretical variance	∞	75.3

Response is the number of normal blastocysts from 12 two-cell ova after three days cultivation in vitro. Two-cell ova obtained 45 hours after HCG injection.

¹0.05 > P > 0.01.

³P < 0.001.