Figure 4.

Structural mapping of 5′-ATTTUATTT-3′ binding to A3B-CTD. (A) Residues whose resonances experience significant ¹H,¹⁵N chemical shift changes upon binding are colored red (>0.050 p.p.m.) or orange (0.028–0.050 p.p.m.). ¹H,¹⁵N-combined chemical shift changes were calculated using $\sqrt{\mathrm{\Delta }{{\delta }_{HN}}^2+{(\mathrm{\Delta }{\delta }_N/6)}^2}$, with Δδ_HN and Δδ_N representing ¹HN and ¹⁵N chemical shift differences, respectively. (B) and (C) Binding isotherms for representative ¹HN resonances of A3B-CTD (B) and A3A (C).