FIG. 4.

Analysis of decellularization perfusate. (A) Cumulative DNA content of the perfusate after each stage shows the greatest amount of DNA is perfused out after 0.01% SDS perfusion. (B) Total protein analysis of the perfusate demonstrates that most proteins are expelled after the perfusion with PBS and slowly decreased after higher SDS concentrations. (C) Cumulative GAG shows that a lot of GAGs are removed from the ECM throughout the decellularization process with the most prominent removal happening during 0.1% SDS perfusion. All error bars represent SEM (n = 4). Color images available online at www.liebertpub.com/tec