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Histological architecture of BP following antigen removal using chaotropes. H&E staining (20 × ) and VVG staining (20 × ) reveal loss of BP collagen waviness and greater ECM compaction with increasing urea and thiourea concentrations. Elastin molecules fragment and decrease with increasing urea and thiourea concentrations. Scale bar = 100 μm. ECM, extracellular matrix. H&E, hematoxylin and eosin; VVG, Verhoeff van Gieson.
