Fig. 7.
Immunohistochemical analysis of T cell infiltration after treatment of EAE with or without Dex and/or Plerixafor®. C57Bl/6 mice were immunized with MOG35–55 peptide and at day 8 they were either implanted with an osmotic minipump which delivered Plerixafor® over a 14-day period or sham operated as a control. After the onset of first disease symptoms mice receiving Plerixafor® and control mice were further randomly divided into two groups: one of them received 100 mg/kg Dex on three consecutive days and the other one received PBS (corresponding to the experiment shown in Fig. 6a). Spinal cords were obtained at the end of the experiment and analyzed by immunohistochemistry using an anti-CD3 antibody. One representative staining out of four of each group is depicted as an overview of the whole spinal cord (left side) and an enlarged picture taken from the upper middle part (right side). The length of the bar corresponds to 500 or 200 μm, respectively