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. 2016 Jul 13;11(7):e0158967. doi: 10.1371/journal.pone.0158967

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© 2016 Suzuki et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) Retention of FAM-labeled CHST15 siRNA within the submucosa of the normal mouse colon by differential interference contrast microscopy. Arrow indicated the site of injection. FAM-positive cells were clearly detected at higher magnifications (open squares). Original magnification, x100. (B) Diffusion of FAM-labeled siRNA in the whole normal mouse colon by stereoscopic microscopy. Lower panel showed fluorescence signals. Arrow indicated the site of injection. Non-injected colon was placed as a negative control. Original magnification, x7.3. (C) Concentrations of CHST15 siRNA in the normal mouse colon 4, 24, 48 and 144 h after a single submucosal injection.