Table 1. The amounts of CS in condition medium of siRNA-treated colon fibroblasts.
pmol/mg protein (mol%) | ||
---|---|---|
Negative control siRNA | CHST15 siRNA | |
ΔHexUA-GalNAc | 314 ± 40 (2.6 ± 0.3) | 441 ± 47 (3.7 ± 0.4) |
ΔHexUA-GalNAc(6S) | 2,780 ± 189 (22.8 ± 0.8) | 2,640 ± 122 (21.5 ± 0.7) |
ΔHexUA-GalNAc(4S) | 8,045 ± 189 (66.2 ± 0.9) | 8,542 ± 97 (69.8 ± 0.3) |
ΔHexUA(2S)-GalNAc(6S) | 226 ± 15 (1.9 ± 0.1) | 230 ±13 (1.8 ± 0.1) |
ΔHexUA(2S)-GalNAc(4S) | 128 ± 21 (1.0 ± 0.2) | 196 ± 27 (1.6 ± 0.2) |
ΔHexUA-GalNAc(4S,6S) | 672 ± 57 (5.5 ± 0.3) | 196 ± 6 (1.6 ± 0.1) |
Total CS disaccharide | 12,165 ± 419 (100) | 12,244 ± 173 (100) |