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(A) MSP analysis of BRCA1. U: PCR product amplified by unmethylated-specific primers, M: PCR product amplified by methylated-specific primers. (B) BRCA1 protein is expressed in nuclei of normal tissue but drastically diminishes from hyperplasia to SCC. (C) Grade distribution of nuclear expression. **p<0.01. Scale bar: 50 μm.
