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. 2016 Jul 13;11(7):e0159246. doi: 10.1371/journal.pone.0159246

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© 2016 Khoa et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Construction of the Fgf5-P2A-Venus BAC Tg. The Fgf5 BAC clone (RP23-153I24) covering 72 kb upstream and 112 kb downstream of Fgf5 gene was used. Note that the PGK-gb2-neo cassette was removed from the BAC construct prior to generation of Tg mice. P2A: porcine teschovirus-1 self-cleaving peptide; ipac: ires (internal ribosome entry site)-puromycin resistant gene. (B, C) Venus expression in WT and Tg embryos at E6.5 (line #2 and #571). Ex: extraembryonic region; Em: embryonic region. Scale bar: 100 μm.