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. 2016 Jul 13;11(7):e0159246. doi: 10.1371/journal.pone.0159246

Fig 3. Venus expression in Fgf5-P2A-Venus BAC Tg embryo at E7.5.

Fig 3

(A) Immunofluorescence images of the Tg embryo at E7.5 for Oct3/4 (red), Venus (anti-GFP, green), T (purple) and Nuclei (Hoechst33342, blue). Higher magnification of optical sections is shown in panels X and Y. Note that Oct3/4 and Venus were co-expressed in the epiblast of the Tg embryo, while T expression was observed in the mesodermal layer of the Tg embryo. Venus expression was also seen in visceral endodermal layer. Open and closed arrowheads indicate mesodermal and epiblast cells, respectively. Ex: extraembryonic region; Em: embryonic region; Epi: epiblast; Me: mesoderm; T: Brachyury/T. Scale bar: 50 μm. All images were captured by a Leica TCS-SP8 confocal microscope using a 20x/0.7 dry objective lens (projection images) and 40x/1.25 oil objective lens (section, X and Y images). (B) Whole-mount fluorescence in situ hybridization of Fgf5 in the Tg embryo at E7.5. Open arrowheads indicate cytoplasmic localization of endogenous Fgf5 mRNA. Scale bar: 50 μm.