Abstract
Immunoglobulin (Ig) was demonstrated in paraffin sections of 12 trephine bone marrow biopsies by means of the unlabelled antibody peroxidase-antiperoxidase (PAP) method. The Ig-containing cells, which were counted with the Reichert-Jung (Kontron) MOP-AMO3 user-controlled image-analyser, were found to constitute approximately 4·2% of all the nucleated cells in the marrow, a figure significantly higher than those reported by previous workers.
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Selected References
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- Crocker J., Curran R. C. A study of nuclear diameters in lymph node imprints using the Zeiss Microvideomat. J Clin Pathol. 1979 Jul;32(7):670–674. doi: 10.1136/jcp.32.7.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curran R. C., Gregory J. Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections. J Clin Pathol. 1978 Oct;31(10):974–983. doi: 10.1136/jcp.31.10.974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curran R. C., Gregory J. Effects of fixation and processing on immunohistochemical demonstration of immunoglobulin in paraffin sections of tonsil and bone marrow. J Clin Pathol. 1980 Nov;33(11):1047–1057. doi: 10.1136/jcp.33.11.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Langston C., Thurlbeck W. M. The use of simple image analysers in lung morphometry. J Microsc. 1978 Sep;114(1):89–99. doi: 10.1111/j.1365-2818.1978.tb00119.x. [DOI] [PubMed] [Google Scholar]
- Scott K. W., Hoy J. The cross sectional area of diaphragmatic muscle fibres in emphysema, measured by an automated image analysis system. J Pathol. 1976 Oct;120(2):121–128. doi: 10.1002/path.1711200208. [DOI] [PubMed] [Google Scholar]
- Slavin G., Sowter C., Robertson K., McDermott S., Paton K. Measurement in jejunal biopsies by computer-aided microscopy. J Clin Pathol. 1980 Mar;33(3):254–261. doi: 10.1136/jcp.33.3.254. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turesson I. Distribution of immunoglobulin-containing cells in human bone marrow and lymphoid tissues. Acta Med Scand. 1976;199(4):293–304. doi: 10.1111/j.0954-6820.1976.tb06735.x. [DOI] [PubMed] [Google Scholar]